Senior Scientist I - Protein Engineering

OPPORTUNITY

Mammoth Biosciences is seeking a Senior Scientist I for its Protein Engineering team. This role will execute design-build-test-learn cycles to create and optimize proteins for gene editing applications.The successful candidate will be responsible for proposing and executing strategies for high-throughput generation and screening of proteins involved in CRISPR systems for gene editing applications. This role will function as part of the Protein Engineering group, which is responsible for optimizing and creating proteins across all gene editing modalities being pursued at Mammoth Biosciences.

KEY RESPONSIBILITIES

• High-throughput molecular cloning and diversity generation for protein engineering targets 
• High-throughput screening in mammalian systems for increased potency and specificity in gene editing outcomes
• Design and use of both pooled and arrayed screening strategies for efficient identification of efficacious variants and fusion proteins
• Use of NGS and FACS assays for ascertaining editing outcomes 
• Identification of structure-activity relationships from engineering outcomes
• Nomination of key molecules for downstream testing by project teams 
• Working with editing technology project teams to optimize new gene editing modalities 
• Analysis of large datasets
• Documentation of experimental results in laboratory notebooks, present experimental results at internal meetings, and other duties as assigned

REQUIRED QUALIFICATIONS

• Bachelors, Masters, or Ph.D. in Biochemistry, Bioengineering, Molecular Biology, or related area
• Postgraduate experience in years (industry/postdoc): Bachelor's 8+, Masters 6+, or PhD 3+ 
• Expertise in high-throughput methods for protein diversity generation 
• Expertise in arrayed and pooled (e.g. deep mutational scanning) screening approaches in mammalian cells with NGS and FACS-based readouts
• Experience in constructing reporter cell lines for FACS-based screens
• Experience with analysis of large datasets

PREFERRED QUALIFICATIONS

• Familiarity with liquid handlers such as Hamilton/Tecan/ECHO
• Familiarity with colony pickers such as Qpix
• Experience with CRISPR systems and/or other gene editing technologies
• Experience leading and mentoring scientists and collaborating cross functionally
• Computer and scripting skills (Benchling, Geneious, JMP, Python, FlowJo)

BENEFITS

• Company-paid health/vision/dental benefits
• Unlimited vacation and generous sick time
• Company-sponsored meals and snacks
• Wellness, caregiver and ergonomics benefits
• 401(k) with company matching

Base Salary Range: $ 150,000 - $165,162

Actual base salary will be determined by relevant professional experience, applicable skills, and internal equity.

COMPANY

Mammoth Biosciences is a biotechnology company focused on leveraging its proprietary ultracompact CRISPR systems to develop long-term curative therapies, as well as other applications such as decentralized precision diagnostics. Founded by CRISPR pioneer and Nobel laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington, the company’s ultracompact proteins are designed to enable in vivo gene editing in difficult to reach tissues utilizing both nuclease applications and new editing modalities beyond double stranded breaks, including base editing, gene writing, and epigenetic editing. The company is building out its pipeline of potential in vivo gene editing therapeutics and capabilities and has partnerships with leading pharmaceutical and biotechnology companies to broaden the reach of its innovative and proprietary technology. Mammoth’s deep science and industry experience, along with a robust and differentiated intellectual property portfolio, have enabled the company to further its mission to transform the lives of patients and deliver on the full promise of CRISPR technologies.

It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation.

Mammoth Biosciences requires that all employees be vaccinated against COVID-19 as a condition of at-will employment, with exceptions for medical or religious reasons in compliance with local, state, and federal law.